I believed this was accurate, but upon reflection, using a pipette would have been much more accurate as pipettes have a much lower apparatus error than volumetric flasks.
This should tell me whether the reaction is indeed a first-order reaction, or if a curve of best fit is required. So in cm3, the actual volume could have been either Consistent Temperature Substrate concentration coursework is a major factor which affects the rate of reaction.
This would have affected the number of molecules of hydrogen peroxide present, which in turn would have affected the number of collisions between enzyme and substrate molecules. The line of best fit also suggests values of concentrations which I have not investigated.
However, there are some factors that I must take into consideration. As I have mentioned earlier, this decreases in steps of 3. Another factor which was hard to measure was the volume of gas produced, because some of the higher concentration reactions were very fast, so it was hard to read the correct values every time.
I solved this by swirling the conical flask constantly throughout the reactions, which seemed to solve the problem, although this meant that the amount of swirling had to be the same in order Substrate concentration coursework ensure a fair test.
A larger surface area means there are more molecules being exposed to collisions with other molecules, with sufficient energy to cause a reaction. I will then work out the gradient of the two concentrations and plot them on a rate of reaction graph along with the other concentrations.
Apparatus Limitations Firstly, there were limitations on the apparatus that I used. Average final volumes of oxygen for each concentration of hydrogen peroxide. This is because the molecules of both substrate and enzyme have more energy, therefore they collide more often and produce more heat energy.
I will use exactly the same method as I did previously, and since I still have some yeast left, I can still use the same batch of yeast. It was very hard to insert the small 5cm3 beaker into the conical flask, and when it came to tipping it over, some of the substrate was still trapped inside the beaker.
The higher the concentration, the more heat will be produced. This means the amount of substrate could have been different for each repeat, even though I used the same concentration.
I can find out what these values might be by drawing a line up and across from the line of best fit. I have drawn a line of best fit to clearly illustrate this trend. If the line of best fit is correct, it would make this value an anomaly, which clearly it is not since it is the most accurate value on the graph.
To prevent this I had to dry out the barrel and syringe before commencing the procedure. Clean and Dry Equipment I also had to make sure I washed out the conical flask and beaker thoroughly with distilled water and dried them sufficiently.
Because the volume of oxygen dissolved in the reaction is not constant for all reactions, and less oxygen is dissolved in water at higher temperatures, this would have affected my results.
This means that having the same surface area of yeast in each reaction is very important in ensuring a fair test because the number of molecules exposed to collisions must be the same.
Now that I have carried out the repeats and plotted the points on the rate of reaction graph, I can see that the graph is in fact clearly linear. For example, if there were more molecules of yeast, the rate of reaction would increase because there would be more collisions between enzyme and substrate molecules.Substrate Concentration.
So how many scrambled eggs can you make? Well, that depends on the number of eggs you have.
The amount of eggs is your concentration. Substrate concentration is the amount of substrate present that can be turned into product.
The. Coursework Writing Service; Essay Outline/Plan Service; Enzyme Activity As A Function Of Substrate Concentration Biology Essay.
Print Reference this. Published: 23rd March, Enzyme activity is generally greatest when substrate concentration is unlimiting. Apr 30, · The substrate concentration at this point, even if increased, will not affect the rate of reaction because it is the enzyme which is in low concentration.
Draw a graph showing what your PREDICTION will be, and write a statement (such as the one Reviews: 5. - Effect of Substrate Concentration on Catalase Aims This is an experiment to examine how the concentration of the substrate Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase.
GCSE Chemistry Coursework Investigation] Powerful Essays words | ( pages) |. Initially FREE enzyme concentration falls, substrate binds to it and the concentration of complex rises, steady state is achieved NOT EQUILIBRIUM (overall concentration of substrate is falling with time, generation of product, product concentration increases, and complex of enz-sub does not change).
Experiment 2: The Effect of Substrate Concentration on Catalase Activity Lab Results 1. Fill in the table below with your results from the substrate concentration experiment.
Substrate Concentration Results Test Tube Bubbles Formation (min) 1 started right away for about 4 min 2 started right away for about 4 min 3 started right away for about 5 min 4 started right away for about 5 min Data Analysis %(52).Download